Ng Ul To Molarity Calculator. Do I use beer-lamberts law or calculate concentration from ng/ul

Do I use beer-lamberts law or calculate concentration from ng/ul concentration using molecular weight of the oligos? Both calculations give me VERY different results for concentration. (Molar concentration) If you use roughly 2 ul of your vector (at 150 ng per ul), then that is a good amount of vector for 1 ligation. Ideal for scientific labs and industrial applications. Sep 18, 2025 · Ng/ml to molarity is a conversion process used in chemistry to change the concentration of a solution from nanograms per milliliter (ng/ml) to molarity (M). In the same way the program will recalculate answer after changing of other parameters (weight, volume, size). You should obtain 254. Oct 1, 2025 · Enter the molarity, concentration, and molecular weight into the calculator to determine the missing variable. For double-stranded DNA (dsDNA), the average molar mass per base pair (bp) is approximately 650 g/mol. When we pool libraries for NGS, the best practice is to use the molarity of each library. Illumina Miseq concentration calculation: Multiply the nM indicator above for your average size (from the Bioanalyzer) by the Qubit concentration reading (ng/ul) to generate the Illumina converted concentration value for each sample. Dilute solution to a desired concentration (mass/vol). How much is 1 Micromole per liter? Measurement calculator to convert umol/l. , Units/mg). To convert from ng/µl to nM for cluster generation, follow the instructions From that you can convert to ng/uL for a 1 M solution. Accurate, free, and fast for lab professionals and researchers. m is the mass (i. Molarity is a measure of the number of moles of a solute per liter of solution, while ng/ml measures the mass of the solute per volume of solution. Optionally calculates A260/A280 ratio. 3pM. DNA/RNA Molarity Calculator PRE-CLINICAL RESEARCH SERVICES: Pharm/Tox Testing, IC50 for 100+ Cancer Cell Lines 80 Xenograft Models: Anti-Tumor Activity Test In Vivo Liposome Encapsulation Services: siRNA, mRNA, DNA ELISA and Cell-Based Assay Development GENERATION OF STABLE CELL LINES: Generation of Stable Cell Line in 28 Days Unit of Measure for Library nM ng/µl Pooled Library Concentration (nM) Total Pooled Library Volume (µl) Description (optional) The mass molarity calculator tool calculates the mass of compound required to achieve a specific molar concentration and volume. g. I have a qubit measurement of my DNA library at 25ng/ul and the average… What is a Reconstitution Calculator? Definition: This calculator determines the reconstitution concentration of a substance (e. , aliquoted) from the concentrated stock solution. V2 is the final volume of the diluted solution. Jan 13, 2021 · In order to convert from a mass/volume (concentration) to a molarity, you need to use the g/mol of the molecule in question. DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. Object Moved This document may be found here Calculating the optimal amounts of vector and insert to use in the In-Fusion Cloning reaction is easy with our In-Fusion molar ratio calculator—simply provide the size of your insert and vector in base pairs below. As a result, we often have to convert a concentration measured in ng/μl into nM and then perform a dilution to create a solution containing the desired number of nanomoles. Then combine this with 15-0. What I don't understand is why we use molarity and not concentration. This page provides unit conversion tools for nanogram per microliter (ng/uL), a concentration measurement. Analyze, graph and present your scientific work easily with GraphPad Prism. This value can be adjusted for different DNA types or organisms. , a medication or chemical) based on the dose and the volume of the solvent used for reconstitution. Dilute solution to a desired Molarity. Mar 26, 2025 · Converting nanograms per microliter (ng/µL) to molarity (M) is a fundamental calculation in biochemistry and molecular biology. e. I have been out of the lab for sometime but I need to do a sequencing run. Apr 9, 2005 · Dilution Calculator - Mass per Volume (Mass / Volume) C1 is the concentration of the stock solution. Perform precise NG UL dilution calculations effortlessly with this accurate calculator. Apr 30, 2005 · C is the desired concentration of the final solution with the concentration unit expressed in units of activity per volume of solution (e. This calculator determines the dilutions needed to obtain target template concentrations in nM from samples whose length in base pairs (bp) and concentration in ng/μl is Ligation Calculator This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. Anybody can That's correct, though I would just calculate (10 ng)/ (937 ng/ul) to get 0. V1 is the volume to be removed (i. Or view the worksheet. molar quantity (mol) --> Molar concentration (M) Simplify molarity calculations for proteomics, cell biology, and chemistry. Library Size (bp) Description (Optional) Library Concentration (ng/µl) Library Concentration (nM) Library 1 Convert Concentration Copy Calculations They support critical tasks such as molarity adjustments, concentration conversions, and precise dilution ratio determinations. Cortisol conversion calculator for units in scientific, laboratory and medical reports. Calculate the molar mass of a gas at 369 torr and 72°C if 206 ng occupies 0. These methods typically measure dsDNA concentration in ng/µl. This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for nucleic acids, and vice versa. torr and 100. You'll need to dilute your insert a lot though. Dilution Calculator This tool will calculate the amount of stock solution needed to achieve a desired concentration in a given volume. » Molar Mass Calculator » Cations, Anions List » Dilution Calculator » Molarity Calculator » Compound Quiz » Concentration Solution Unit Converter Home Unit Conversions Biology Geometry, Trigonometry Physics Chemistry Mathmatics Medical Algebra Statistics English words R Language Tutorials HTML Tutorials Time Zone Converter endmemo. New! The In-Fusion molar ratio calculator is now optimized for multi-insert cloning. What is a Reconstitution Calculator? Definition: This calculator determines the reconstitution concentration of a substance (e. Library Concentration Conversion Calculator Consider the xGen™ Normalase™ Module and xGen Normalase indexing primers for enzymatic normalization of up to 1,536 libraries. Use the Molarity Calculator to calculate the mass, volume or molar concentration required to prepare a solution of compound of known molecular weight. °C if 206. g/mol Serial Dilution Calculator for 2-fold, 4-fold, CFU, percent, and ELISA dilutions. 44 g/mol × 1000000 = 8. Can anyone tell me the formula to achieve required ratios within 1. . Below are calculations to convert between these units. The most useful formula you will be using is: M 1 V 1 = M 2 V 2, Starting molarity (M 1) multiplied by starting volume (V 1) equals the desired molarity (M 2) multiplied by the desired volume (V 2). A is the activity of the solid material with the activity unit expressed in units of activity per mass of material (e. Whether you start with an OD₂₆₀ reading, a mass concentration, or a molar value, this tool quickly calculates the equivalents. It also recalculates grams per ml to moles. So if you use Qubit to measure your libraries, you have to convert ng/uL to nM. Molarity Calculator Molarity is short for molar concentration, which is a common measurement of concentration depending on temperature. C2 is the final concentration of the diluted solution. Convert DNA concentration (ng/µL, µg/mL) to molarity (mol/L, µM, nM) and molecules/µL. Now, push "Calculate" in "Calculation of the molar concentration". double strand DNA base weight is 650 Dalton, for single strand is 330, for RNA is 340 Dalton. Simply select your nucleic acid type (dsDNA, ssDNA, or RNA) and input your known value along with its unit (e. This guide explains the process step-by-step, helping researchers and students prepare accurate solutions for experiments. Dilution calculator of mass concentration or molar concentration, PPM PPB Percentage calculation, Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. Ligation Calculator This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. This calculator is useful for diluting DNA samples. 5ug of total amount of DNA? Calculate the molar mass of a gas at 493. Through this calculator, you can get the result easily after entering any three of these four parameters. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. I have some samples (total RNA) and measured them with nanodrop (1652 ng/ul). , micromolar (µM, 10 -6 M) or nanomolar (nM, 10 -9 M). Simply choose the number of inserts (up to 5). All parameters of the equation can be calculated (solution concentration, solute mass, solution volume, and solute molecular weight). Primer Resuspension Primer information sent from a company will list the molecular weight of your primer (g/mol) and the amount of nmoles that they Convert DNA concentration (ng/µL, µg/mL) to molarity (mol/L, µM, nM) and molecules/µL. They support critical tasks such as molarity adjustments, concentration conversions, and precise dilution ratio determinations. The molarity would be calculated as follows: Molarity = 5000 ng/mL 58. Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. Molar Mass: The molar mass of a DNA fragment is the mass of one mole of that fragment. , weight) of solute that must be dissolved in volume V of solution to make the desired 本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为： 质量 (mg) = 浓度 (mM) × 体积 (mL) × 分子量 (g/mol) Mar 1, 2001 · Use this calculator to determine the molar concentration (i. By entering specific parameters—like initial concentration, final concentration, volume requirements, and dilution factor—users receive accurate dilution measurements tailored to their laboratory and industrial needs. What is the purpose of calculating molarity, diluting everything to 4nM, and combining equal volumes? Cortisol conversion calculator for units in scientific, laboratory and medical reports. This is the volume that results after V1 from the stock solution has been diluted with diluent to achieve Oct 1, 2025 · All Unit Converters All Medical and Health Unit Converters Ph To Hydrogen Ion Concentration Calculator Ng/Ml To Molarity Calculator Ng/Ml To Nmol/L Calculator Um To Ug/Ml Formula The following formula is used to calculate the concentration in micrograms per milliliter (ug/ml) from units of mass (um). 57 × 10 5 mol/L So the molarity of this solution would be 8. (Molar concentration) This calculator converts the mass concentration of any solution into molar concentration. 44 g/mol (like sodium chloride, NaCl). 6 mM (10^-3 molar) This converter is used to convert the molar quantity into the molar concentration, and vice versa. Used to determine how […] Sep 10, 2025 · Ng/ml to Nm is a conversion from nanograms per milliliter to nanomolar. I g mol x16 Х s I know that the concentration is given by the nano drop in ng/UL in the right. You can divide your 1284. Molarity Calculator This tool will calculate the mass needed to generate a solution based on the desired molarity, MW, and volume, or the molarity based on MW, volume, and mass. Mar 15, 2024 · The DNA Dilution Calculator helps scientists and researchers accurately dilute their DNA samples to a desired concentration. Dec 1, 2022 · How to convert ng/ul to nM (ssRNA)? I have a solution of ssRNA molecules and want to know how to convert the ng/ul concentration to nM (nano moles). Jun 2, 2018 · I want to treat my cell with 10, 25 and 50 ng/ml concentration (500 ul each) but I cant figure how to calculate the amount of reagent that need to be used. Nov 9, 2016 · With Promega BioMath Calculator you can obtain, easy and fast that info. 99 ul of water. Units conversion: nmol/L, µg/L, µg/dL, µg/100mL, µg%, ng/mL . This calculator is useful for diluting primers and DNA oligos. Normally, our concentrations are given in units of milligrams per milliliter (mg/ml), but often data sheet quality assurance data are expressed in molarity (moles per liter, M) or fractions of that — e. Chemical / Molarity Calculator You can use this calculator to: Calculate the mass or volume required to prepare a chemical solution that contains a specified molar concentration of a substance. Round your answer to 3 significant figures. At this stage if you know the concentration in ng/uL and the MW, you don't need to use the EC for anything to determine the micromolar This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μl If you know that the weight of your DNA (molar mass per bp) is different from the average weight of DNA basepair, change the value. Oct 1, 2025 · All Unit Converters All Medical and Health Unit Converters Ph To Hydrogen Ion Concentration Calculator Ng/Ml To Molarity Calculator Ng/Ml To Nmol/L Calculator Um To Ug/Ml Formula The following formula is used to calculate the concentration in micrograms per milliliter (ug/ml) from units of mass (um). Reconstitution Calculator Calculate the volume of solvent required to reconstitute your vial The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Nucleic Acid Concentration & Purity Converter Convert between OD₂₆₀, mass (ng/µL, µg/mL, µg/µL), molarity (µM, pmol/µL, nM), and copy number for dsDNA, ssDNA, or RNA. Free online protein molarity calculator - calculate protein molarity enter the concentration and molecular weight of protein then click the calculate button. 8 ng/uL sample concentration by this value to get the molarity of your solution, in M. The formula is related to molarity, mass, volume and molecular weight. Jun 25, 2024 · This is typically expressed as copies per nanogram (copies/ng). Otherwise just use the default value. Easy DNA molarity calculator with formula, chart, and export options. Converting ng/µl to nM when calculating dsDNA library concentration Some standard Illumina libraries, such as Nextera, require the use of dsDNA-specific fluorescent dye methods for accurate quantification. 01=14. This is often used in scientific research, particularly in the fields of chemistry and biology, to convert between these two units of concentration. For comparison of pure biomolecular samples versus heterogeneous samples, dsDNA, protein, and heterogeneous samples were made using stocks solutions of dsDNA (100 ng/μL) and BSA (625 ng/uL). 206 ul. It is also possible to calculate the mass of any substance required to reach a desired level of molarity. com Basic conversion question (ng/ul to ng) Hi all, I know it is a basic calculation and a very dumb question but how to I get an absolute amount (ng) from a concentration (ng/ul). But I want to calculate the concentration by my own with the next information given. Then you can convert from M to uM. To convert from ng/µl to nM for cluster generation, follow the instructions The concentration of plasmid A and B is 580 ng/ul and 1265 ng/ul, respectively. , molarity) of a solution. ng occupies 0. This free molarity calculator determines any of the four variables in the molarity equation, given the other three. DNA/RNA molarity Calculator: M = w / MW Where: M: DNA molarity, in mol w: DNA weight, in g MW: DNA molecular weight, in g/mol If only DNA length is given, the molecular weight is calculated as: MW = DNA Length (bp) × DNA/RNA base weight The approx. Get fast, accurate results for your lab experiments! The mass molarity calculator tool calculates the mass of compound required to achieve a specific molar concentration and volume. No coding required. Dilution: Dec 28, 2024 · Online DNA Calculator: Determine molar concentration, DNA quantity, and dilution requirements in seconds Enter your answer in the provided box. Multiplying by 10^6 is just to get back to the right order of magnitude (nanomolar) since you've just converted in grams per mol Ligation Calculator This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. 01 ul (after rounding off). In addition to concentracion (molarity) you have, you will need the number of bases of your primer. Includes dilution, purity ratios (260/280, 260/230), and step-by-step explanations. Oct 3, 2024 · Yes, this calculator can be used for any dilution where concentrations are expressed in ng/µl, though the method applies to any unit of concentration as long as units are consistent. Note: Reference the Conversion factors for non-SI units, Fundamental constants and SI prefixes tables for additional information. Choose the organism you DNA originates from, or choose "Custom DNA fragment". , Units/mL). LabConvert lets you calculate molarity effortlessly using PubChem data for molecular weights. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs. Change volume for 100µl and just click somewhere outside the field (or, again, push "Calculate"): program will give "218. 7pM". 395,997 / 1000 = 395,9 ul (It is the final volume, but you have 310,1 so, you need add 85,9 ul of TAE or Nuclease Free water to obtain a concentration of 1000 ng/ul). I need to send them for sequencing and I am asked the total amount in ng. Dilution calculator of mass concentration or molar concentration, PPM PPB Percentage calculation, Solutions Calculator: Molecular Weight to Molarity MW or Molar Mass of Solute g/mol Desired Molarity Desired Volume Solution Dilution Calculator Dilute a stock solution of known concentration or molarity The American Elements solution dilution calculator is a tool to help determine the volume of a solvent required to yield a solution of given volume and concentration (molarity) of a fixed amount of solute. , OD₂₆₀, ng/µL, µg/mL, µM, pmol/µL, copies/µL). Example Let's say we have a solution with a concentration of 5000 ng/mL, and the molar mass of the substance is 58. 57 × 10 5 mol/L. Use our DNA concentration calculator to convert A260 readings to ng/µL for dsDNA, ssDNA, or RNA, and convert between mass and molar concentration (nM) by fragment length. Conversely, you can calculate the molarity of a solution prepared by dissolving a certain amount of a substance in a specified volume of a solvent. Molarity Calculator Equation: For example, if we have a 850 ng/ul (=850 mg/l) of DNA, then the molarity of nucleotides equal: 850/327=2. If you choose a custom Molarity refers to the molar concentration of a solution, that is, the number of moles of solute dissolved in 1 liter of solution, as mol/L, abbreviated as M. The mass molarity calculator tool calculates the mass of compound required to achieve a specific molar concentration and volume.

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